British Transport Police were called to a level crossing north of Leominster, Herefordshire, at 10.40am on May 22 following reports that a train had struck a tractor and trailer.
Officers attended alongside officers from West Mercia Police, paramedics and fire service.
Following medical assessments two people were treated for injuries that are not considered to be serious.
Enquiries remain ongoing into the full circumstances leading up to the incident.
Affecting services between Manchester and Cardiff via Hereford, Abergavenny, Pontypool.
A joint statement between Transport for Wales and Network rail said: “The 8.30am Manchester Piccadilly- Cardiff Central service has hit a trailer at a crossing between Ludlow and Leominster, and this will cause disruption along the Marches Line for the remainder of the day.”
“Our immediate priority was the safety of our customers and colleagues. All passengers have been successfully moved from the site.”
“Disruption is expected until the end of the day and we’re advising everyone to check before they travel.”
“Ticket acceptance is in place with Northern, Avanti, Cross Country, West Midlands Railway and on the X24,X3,21 and 23 provided by Stagecoach.”
An update from Transport for Wales and Network Rail said: “The mainline between Hereford and Shrewsbury will remain closed tomorrow following a collision between a train and a tractor trailer on the track near Leominster.”
“TFW and Network Rail are working together to re-open the line and restore services as quickly as possible.”
“Replacement and road transport remains in place between Hereford and Shrewsbury and tickets will also be valid for use with other operators via all reasonable alternative routes as well as with Stagecoach bus.”
“Customers should continue to check before they travel and we will look to provide a further update at midday tomorrow.”
“We’d like to thank everyone for their patience and understanding whilst we safely bring back our services.”